Overview

- Millipore offers four different Immobilon PVDF membranes, each optimized for different protein blotting applications. 

Immobilon PVDF membranes provide many advantages compared to nitrocellulose. They won’t crack or curl, and they can be cut without fracturing. They also have low background, broad solvent compatibility, and superior staining capabilities. In addition, they can be reprobed multiple times.

Immobilon-P Transfer Membrane:

- 45 µm pore size Recommended for most western blotting, especially proteins greater than 20 kDa 

- Millipore's Rapid Immunodetection Protocol reduces detection times by up to 2 hours by eliminating membrane blocking and several wash steps with no loss of sensitivity or specificity 

Immobilon-E Membrane:

- the only PVDF membrane that wets out in aqueous buffers, eliminating the methanol pre-wet step.

- 45 µm pore size recommended for most western blotting, especially proteins greater than 20 kDa 

Blotting Sandwiches:

- When you need to process multiple blots, blotting sandwiches are a convenient, time-saving choice. Our sandwiches include sheets of Immobilon-P membrane interleaved with pre-cut sheets of chromatography-grade blotting filter paper and are compatible with any standard wet transfer device.

Blotting Filter Paper: