X-连锁凋亡蛋白抑制因子(XIAP)重组蛋白

YB606Mu012
Recombinant X-Linked Inhibitor Of Apoptosis Protein (XIAP)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
X-连锁凋亡蛋白抑制因子(XIAP)重组蛋白[ PROPERTIES ]
Residues: Met1~Ser497
Tags: Two N-terminal Tags, His-tag and T7-tag
Accession: P98170
Host: E. coli
Subcellular Location: Cytoplasm, Nucleus.
Purity: >90%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% trehalose, 0.01% sarcosyl.
X-连锁凋亡蛋白抑制因子(XIAP)重组蛋白Predicted isoelectric point: 6.2
Predicted Molecular Mass: 60.4kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
MTFNSFEGSK TCVPADINKE EEFVEEFNRL KTFANFPSGS PVSASTLARA GFLYTGEGDT
V R C F S C H A AV DRW Q Y G D S AV GR H R K V S P N C RFI N G F Y L E N SAT Q S T N S G I
QNGQYKVENY LGSRDHFALD RPSETHADYL LRTGQVVDIS DTIYPRNPAM YSEEARLKSF
X-连锁凋亡蛋白抑制因子(XIAP)重组蛋白QNWP DYA H LT PRE LAS AG LY YTGIG DQV QC FCC G GK LK NW EP C DRAWSE H
RRHFPNCFFV LGRNLNIRSE SDAVSSDRNF PNSTNLPRNP SMADYEARIF TFGTWIYSVN
KEQ LA RAGFY ALGEGDKVK C FHCGG G LTDW KPSE DPWEQ H AKWYPGCKY L
LEQKGQEYIN NIHLTHSLEE CLVRTTEKTP SLTRRIDDTI FQNPMVQEAI RMGFSFKDIK
KIMEEKIQIS GSNYKSLEVL VADLVNAQKD SMQDESSQTS LQKEISTEEQ LRRLQEEKLC
KICMDRNIAI VFVPCGHLVT CKQCAEAVDK CPMCYTVITF KQKIFMS